Composite

Part:BBa_K1647006:Design

Designed by: Yun Xue   Group: iGEM15_WHU-China   (2015-09-15)

J23100+B0034+Cph8+B0034+ho1+B0034+pcyA+B0010


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3870
    Illegal XhoI site found at 427
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3423
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used a Cph8 protein, equipped with two assistant gene ho1 and pcyA, for strong response to red light.

Source

Assembled from parts in the previous distribution kits.

References